Event Period: Specialized Training at BARI & Tenure as Assistant Director (TC), DD Office (TC) and Tissue Culture Laboratory, BADC Kashimpur (DEC 2022 – FEB 2023)

Clean, virus-free planting material is the foundation of modern horticultural productivity. Alongside completing intensive Special Training on Plant Tissue Culture at the Bangladesh Agricultural Research Institute (BARI), I served as Assistant Director (TC) at the DD Office (TC) and BADC Tissue Culture Laboratory in Kashimpur, Gazipur (টিসুকালচার এর উপর BARI এর বিশেষ প্রশিক্ষণ এবং BADC টিসুকালচার ল্যাব পরিচালনা).

BADC Tissue Culture Laboratory & Acclimatization Operations In-vitro micropropagation and ex-vitro hardening of disease-free horticultural and seed potato plantlets.

BARI Special Training on Plant Tissue Culture Aseptic laminar-airflow culture transfer, media preparation, and meristem excision protocols at BARI.

Growth Room & Culture Vessel Inspection Monitoring shoot proliferation and root induction under controlled photoperiod and temperature regimes.

Acclimatized Tissue-Cultured Crop Production Evaluating field performance of tissue-culture-derived strawberry, banana, and potato lines at Kashimpur.

Core Laboratory & Production Focus

• Meristem Culture & Virus Elimination: Producing pathogen-free nuclear stock for vegetatively propagated crops including potato, banana, strawberry, and ornamental orchids.
• Culture Media Optimization: Formulating Murashige and Skoog (MS) basal media with precise auxin-to-cytokinin ratios for rapid multiplication and vigorous rooting.
• Primary & Secondary Hardening: Managing the delicate transition of plantlets from 100% humidity culture vessels into sterile cocopeat plug trays inside climate-controlled mist chambers.

Potato Cold Storage & Breeder Seed Tuber Preservation (Kashimpur, Gazipur)

Alongside directing aseptic micropropagation in the BADC Tissue Culture Laboratory (Nov 2022 – Feb 2023), my mandate as Assistant Director (Tissue Culture Laboratory and Potato Cold Storage) included managing industrial cold-chain storage for tissue-culture-derived mini-tubers and foundation seed potatoes—maintaining strict temperature (2–4 °C), relative humidity (90–95%), and CO2 ventilation regimes to preserve tuber dormancy and vigor.

Md. Mamunur Reza

Assistant Program Director (Seed), APCU-BADC PARTNER • PhD Fellow, Atatürk University

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